Original Articles

Rafan Oday Fahad*, Saad Abdulbaqi# & Hassan J Hasony@
*MBChB, MSc Microbiology. #MBChB, CABP, Department of Pathology. @ MPhil, PhD, Department of
Microbiology, College of Medicine, University of Basrah, Basrah, Iraq.
Cervical cytobrush and Pap smears were collected from 103 women attending the outpatient
department at Basrah Maternity and children hospital during the period from October 2009 till
the end of January 2010. DNA was successfully extracted from 91 cytobrush samples, amplified
for the detection of human papilloma viruses (HPVs) using GP5+/GP6+ primers, in addition to
typing using type-specific primers for HPV-16 and HPV-18 genotypes.
The overall HPV prevalence was 20.8% with the dominance of genotype 16 (36.6%) over the
genotype 18 (10.5%) and the presence of non-16, non-18 genotype(s) in 42.1% of all HPV
positive cases. Younger women aged 25 years or less were more infected (26.3%) with the
dominance of genotype-16 (21.1%) and among women aged 36-45 years, the non-16/non-18
genotype(s) were the more frequently observed (13.3%). Infection rates were more frequent
among women married above 30 years (33.3%) and those with more than one lifetime husband
(28.6%). Women's husband with polygamy practice significantly covariate with HPV infections
(P< 0.05).
Significant association (P<0.001) was obvious between infection with any HPV and abnormal
cytology. The non-16/non-18 genotype(s) were more involved (28%) whereas genotype-16 was
detected more frequent (20%) than genotype-18 (4%). In addition mixed infection of both high
risk types (16 and 18) was limited only to women with abnormal Pap smears.
In archival blocks, the presence of HPV- DNA was constant in pre-invasive sequamous cervical
lesions as all blocks revealed a positive HPV-16 either alone or in combination with genotype-