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Assessment of salivary levels of 8-OHdG in patients with periodontitis and/or obesity

Document Type : Original Article

Authors

1 Department of Periodontics, College of Dentistry, University of Baghdad

2 B.D.S., M.Sc. Periodontics/ Assistant Professor/ Periodontist College of dentistry, University of Baghdad, Baghdad, Iraq

Abstract

Background: Periodontitis is universally agreed to be an inflammatory disease that arises as a consequence of the interaction between periodontal pathogens and the host’s immune response, oxidative stress might be one of the most significant contributors to the development and progression of periodontitis. One of the most significant markers of oxidative stress is a molecule called 8-OHdG.
Aim of the study: Analyzing the levels of 8-OHdG in saliva of periodontitis/obese patients in comparison to healthy controls with analysis of its correlation with various clinical periodontal parameters.
Materials and methods: 110 subjects (30 obese, 30 periodontitis, 30 obese periodontitis and 20 healthy subjects) took part in the study. Salivary samples were obtained from all subjects prior to clinical examination. Full periodontal examination was conducted for each participant which entailed plaque index PLI, bleeding on probing BOP, probing pocket depth PPD and clinical attachment loss CAL. Obesity was assessed by using body mass index BMI.
Results: It was showed that periodontitis patients and obese patients had significantly elevated concentrations of salivary 8-OHdG when compared with healthy subjects. Also these levels correlated positively with CAL in obese periodontitis patients.
Conclusions: This study revealed an association between 8-OHdG and periodontitis which suggests that this biomarker plays some part in the pathogenesis of periodontal disease. Moreover, this study showed an association between this biomarker and obesity measures, which suggests that periodontitis and obesity are interconnected via oxidative stress.

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References
1.Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Clin Periodontol. 2018;45 Suppl 20:S162-s70
 
2.Hu F. Obesity Epidemiology: Oxford University Press, USA; 2008.
https://doi.org/10.1093/acprof:oso/9780195312911.001.0001.
 
3.Abas D, Eqbal G. Prevalence of obesity among adolescents at secondary schools in Kirkuk city. Iraqi National Journal of Nursing Specialties. 2018;26:96-101. https://doi.org/10.58897/injns.v26i2.175.
 
4.Ng M, Fleming T, Robinson M, Thomson B, Graetz N, Margono C, et al. Global, regional, and national prevalence of overweight and obesity in children and adults during 1980-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2014;384(9945):766-81
https://doi.org/10.1016/S0140-6736(14)60460-8.
 
5.Jasim HM, Abdul Hussein HM, Al-Kaseer EA. Obesity among females in Al-Sader city Baghdad, Iraq, 2017. Journal of the Faculty of Medicine Baghdad. 2018;60(2):105-7
https://doi.org/10.32007/19jfacmedbaghdad36.v60i2.15.
 
6.Martinez-Herrera M, Silvestre-Rangil J, Silvestre FJ. Association between obesity and periodontal disease. A systematic review of epidemiological studies and controlled clinical trials. Med Oral Patol Oral Cir Bucal. 2017;22(6):e708-e15. https://doi.org/10.4317/medoral.21786.
 
7.Nascimento GG, Leite FR, Do LG, Peres KG, Correa MB, Demarco FF, et al. Is weight gain associated with the incidence of periodontitis? A systematic review and meta-analysis. J Clin Periodontol. 2015;42(6):495-505. https://doi.org/10.1111/jcpe.12417.
 
8.Pataro AL, Costa FO, Cortelli SC, Cortelli JR, Abreu MH, Costa JE. Association between severity of body mass index and periodontal condition in women. Clin Oral Investig. 2012;16(3):727-34
https://doi.org/10.1007/s00784-011-0554-7.
 
9.Gul S, Imran N, Al-Sharqi A, Abdulkareem A. Association of overweight/obesity with the severity of periodontitis using BPE code in an iraqi population. Clinical Epidemiology and Global Health. 2020;9
https://doi.org/10.1016/j.cegh.2020.06.005.
 
10.Ismail H, Mahmood M. Effect of melatonin supplementation on the gingival health and lipid profiles in obese periodontitis patients. Journal of Baghdad College of Dentistry. 2022;34:51-9
https://doi.org/10.26477/jbcd.v34i1.3092.
 
11.Jepsen S, Suvan J, Deschner J. The association of periodontal diseases with metabolic syndrome and obesity. Periodontol 2000. 2020;83(1):125-53. https://doi.org/10.1111/prd.12326.
 
12.Dursun E, Akalin FA, Genc T, Cinar N, Erel O, Yildiz BO. Oxidative Stress and Periodontal Disease in Obesity. Medicine (Baltimore). 2016;95(12):e3136. https://doi.org/10.1097/MD.0000000000003136.
 
13.Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 2007;43:160-232. https://doi.org/10.1111/j.1600-0757.2006.00178.x.
 
14.Paredes-Sánchez E, Montiel-Company JM, Iranzo-Cortés JE, Almerich-Torres T, Bellot-Arcís C, Almerich-Silla JM. Meta-Analysis of the Use of 8-OHdG in Saliva as a Marker of Periodontal Disease. Dis Markers. 2018;2018:7916578. https://doi.org/10.1155/2018/7916578.
 
15.Buczko P, Zalewska A, Szarmach I. Saliva and oxidative stress in oral cavity and in some systemic disorders. J Physiol Pharmacol. 2015;66(1):3-9..
 
16.Trivedi S, Lal N. Antioxidant enzymes in periodontitis. J Oral Biol Craniofac Res. 2017;7(1):54-7
https://doi.org/10.1016/j.jobcr.2016.08.001.
 
17.Henry LG, McKenzie RM, Robles A, Fletcher HM. Oxidative stress resistance in Porphyromonas gingivalis. Future Microbiol. 2012;7(4):497-512. https://doi.org/10.2217/fmb.12.17.
 
18.Tenovuo J, Lagerlof F. Textbook of clinical cardiology. 2nd ed. A T, O. F, editors. Munksgaard Copenhagen: Munksgaard Copenhagen; 1994. 17-43 p..
 
19.Nuttall FQ. Body Mass Index: Obesity, BMI, and Health: A Critical Review. Nutr Today. 2015;50(3):117-28. https://doi.org/10.1097/NT.0000000000000092.
 
20.Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Clin Periodontol. 2018;45 Suppl 20:S149-s61
https://doi.org/10.1111/jcpe.12945.
 
21.Chapple ILC, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol. 2018;89 Suppl 1:S74-s84.
 
22.Podzimek S, Vondrackova L, Duskova J, Janatova T, Broukal Z. Salivary Markers for Periodontal and General Diseases. Dis Markers. 2016;2016:9179632. https://doi.org/10.1155/2016/9179632.
 
23.Almerich-Silla JM, Montiel-Company JM, Pastor S, Serrano F, Puig-Silla M, Dasí F. Oxidative Stress Parameters in Saliva and Its Association with Periodontal Disease and Types of Bacteria. Dis Markers. 2015;2015:653537. https://doi.org/10.1155/2015/653537.
 
24.Sezer U, Ciçek Y, Canakçi CF. Increased salivary levels of 8-hydroxydeoxyguanosine may be a marker for disease activity for periodontitis. Dis Markers. 2012;32(3):165-72. https://doi.org/10.1155/2012/215430.
 
25.Zamora-Perez AL, Ortiz-García YM, Lazalde-Ramos BP, Guerrero-Velázquez C, Gómez-Meda BC, Ramírez-Aguilar M, et al. Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases. J Periodontal Res. 2015;50(1):28-36. https://doi.org/10.1111/jre.12175.
 
26.Miricescu D, Totan A, Calenic B, Mocanu B, Didilescu A, Mohora M, et al. Salivary biomarkers: relationship between oxidative stress and alveolar bone loss in chronic periodontitis. Acta Odontol Scand. 2014;72(1):42-7. https://doi.org/10.3109/00016357.2013.795659.
 
27.Canakçi CF, Ciçek Y, Canakçi V. Reactive oxygen species and human inflammatory periodontal diseases. Biochemistry (Mosc). 2005;70(6):619-28. https://doi.org/10.1007/s10541-005-0161-9.
 
28.Su H, Gornitsky M, Velly AM, Yu H, Benarroch M, Schipper HM. Salivary DNA, lipid, and protein oxidation in nonsmokers with periodontal disease. Free Radic Biol Med. 2009;46(7):914-21
https://doi.org/10.1016/j.freeradbiomed.2009.01.008.
 
29.Takane M, Sugano N, Ezawa T, Uchiyama T, Ito K. A marker of oxidative stress in saliva: association with periodontally-involved teeth of a hopeless prognosis. J Oral Sci. 2005;47(1):53-7
https://doi.org/10.2334/josnusd.47.53.
 
30.Savini I, Catani MV, Evangelista D, Gasperi V, Avigliano L. Obesity-associated oxidative stress: strategies finalized to improve redox state. Int J Mol Sci. 2013;14(5):10497-538
https://doi.org/10.3390/ijms140510497.
 
31.Higuchi M, Dusting GJ, Peshavariya H, Jiang F, Hsiao ST, Chan EC, et al. Differentiation of human adipose-derived stem cells into fat involves reactive oxygen species and Forkhead box O1 mediated upregulation of antioxidant enzymes. Stem Cells Dev. 2013;22(6):878-88
https://doi.org/10.1089/scd.2012.0306.
 
32.Horvath TL, Andrews ZB, Diano S. Fuel utilization by hypothalamic neurons: roles for ROS. Trends Endocrinol Metab. 2009;20(2):78-87.
https://doi.org/10.1016/j.tem.2008.10.003.
 
33.Zalewska A, Kossakowska A, Taranta-Janusz K, Zięba S, Fejfer K, Salamonowicz M, et al. Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents. J Clin Med. 2020;9(2)
https://doi.org/10.3390/jcm9020548.
 
34.Kroese LJ, Scheffer PG. 8-hydroxy-2'-deoxyguanosine and cardiovascular disease: a systematic review. Curr Atheroscler Rep. 2014;16(11):452. https://doi.org/10.1007/s11883-014-0452-y.
 
35.Elwakkad A, Hassan NE, Sibaii H, Zayat Se, Sherif L, Hameed E-R-A, et al. Amany Elwakkad, Nayera Elmorsi Hassan, Hiba Sibaii, Salwa el Zayat, Lobna Sherif, Enas-R-Abdel Hameed and Azza-Abdel Shaheed, 2011. Relationship Between Obesity and 8-hydroxy-2-deoxy Guanosine as an Oxidative Marker in Obese Adolescents of Giza. Journal of Medical Sciences, 11: 231-235. 2011
https://doi.org/10.3923/jms.2011.231.235.
 
36.Storr SJ, Woolston CM, Martin SG. Base excision repair, the redox environment and therapeutic implications. Curr Mol Pharmacol. 2012;5(1):88-101. https://doi.org/10.2174/1874467211205010088.
 
37.Öngöz Dede F, Bozkurt Doğan Ş, Ballı U, Avcı B, Durmuşlar MC. The effect of initial periodontal treatment on plasma, gingival crevicular fluid and salivary levels of 8-hydroxy-deoxyguanosine in obesity. Arch Oral Biol. 2016;62:80-5. https://doi.org/10.1016/j.archoralbio.2015.11.014.
 
38.Canakçi CF, Canakçi V, Tatar A, Eltas A, Sezer U, Ciçek Y, et al. Increased salivary level of 8-hydroxydeoxyguanosine is a marker of premature oxidative mitochondrial DNA damage in gingival tissue of patients with periodontitis. Arch Immunol Ther Exp (Warsz). 2009;57(3):205-11
https://doi.org/10.1007/s00005-009-0026-9.
Basrah Journal of Surgery
Volume 29, Issue 2 - Issue Serial Number 58
Published
December 2023
Page 52-65
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History
  • Receive Date: 05 April 2023
  • Revise Date: 11 June 2023
  • Accept Date: 15 November 2023
  • Publish Date: 30 December 2023
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