Print ISSN: 1683-3589

Online ISSN: 2409-501X

Keywords : ISOLATION OF STREPTOCOCCUS AGALACTIAE


9- ISOLATION OF 9-STREPTOCOCCUS AGALACTIAE FROM WOMEN WITH UTERINE TUMORS

Kudus E Omaro; Haifa ALShaheen; Hadeel T AL-Hadithi

Basrah Journal of Surgery, Volume 15, Issue 1, Pages 53-60
DOI: 10.33762/bsurg.2009.55277

Streptococcus agalactiae was isolated from eight out of 42 cases of uterine tumors and
endometrial hyperplasia from inpatients underwent total abdominal hysterectomy in Basrah
Maternity and Child Hospital, due to irregular vaginal bleeding not responding to medical and
hormonal treatment. Six isolates were from uterine tumors, and two from endometrial
hyperplasia. Isolates were identified by being Gram positive, negative for catalase, giving
positive CAMP test, ability to grow in 6.5% NaCl but not 40% Bile salts and resistance to
Bacitracin.
In vitro studies were conducted to investigate virulence factors of isolated S. agalactiae by
testing their ability to produce haemolysin, yellow pigmentation, capsule, resistance to
tetracycline which is indicative for the existence of sialic acid (antiphagocytosis material).
Measurement of the content of sialic acid and lipotechoic acid revealed differences between
isolates.
All isolates, were able to adhere to plastic surfaces and formation of biofilms in both neutral
(pH=7) and acid (pH=4.5) media. The present study has recorded for the first time, resistance of
four isolates of S. agalactiae (50%) to Vancomycin. This finding is worrisome from the clinical
point of view, as these isolates may become a potential source for transmission of Vancomycin
resistance to other bacteria.