Ali K Jumaa; Sarkis K Strak
Abstract
Helicobacter pylori (H. pylori) is a gram-negative bacterium that specifically colonizes the gastric epithelium causing many complications. The link between H. pylori infection and diabetes mellitus (DM) remains controversial. This study was conducted to determine the frequency of H. pylori infection ...
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Helicobacter pylori (H. pylori) is a gram-negative bacterium that specifically colonizes the gastric epithelium causing many complications. The link between H. pylori infection and diabetes mellitus (DM) remains controversial. This study was conducted to determine the frequency of H. pylori infection among a group of subjects with DM and to compare it with that of an age and sex-matched group of non-diabetic subjects.
This case control study was conducted in Al-Sadr Teaching Hospital, Basrah, Southern Iraq from January 2013 to September 2014 on 200 subjects aged ≥ 40 years, 100 diabetic and 100 non-diabetic subjects. All subjects were required to submit fresh stool samples which were tested for evidence of H.pylori infection by stool antigen positivity.
Helicobacter pylori infection was detected in 43% of diabetic group and 25% of controls, which was found to be statistically significant ( p value=0.007).
In conclusion, the present study suggests that diabetic subjects are at more risk for H. pylori infection in comparison to non - diabetic subjects. This association is found to be higher in those with long duration of DM and those with poor glycemic control.
Sawsan S Al-Haroon; Sarkis K Strak; Rafid Adil Abood
Abstract
This study aimed to assess the accuracy of histopathology, brush cytology, and
urease test in the diagnosis of Helicobacter pylori and to evaluate the effect of test
duration on the sensitivity and specificity of positive urease test for the detection of H.
pylori.
Fifty patients [25 patients with ...
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This study aimed to assess the accuracy of histopathology, brush cytology, and
urease test in the diagnosis of Helicobacter pylori and to evaluate the effect of test
duration on the sensitivity and specificity of positive urease test for the detection of H.
pylori.
Fifty patients [25 patients with gastritis group A and 25 patients with duodenal ulcer
group B] selected from those attending endoscopy unit for dyspeptic symptoms, were
enrolled in the study. Four endoscopic biopsies were taken from each patient. One
biopsy from each of antrum and body were obtained for urease test (Urease test was
read at 30 min, 1, 4 and 24 hour after biopsy insertion into the reagent), and one
biopsy from each of antrum and body were used for histopathological examination.
Antral brush cytology was taken also from each patient. The patients were considered
H. pylori positive when minimum concordances of 2 out of 3 tests (Histopathology,
brush cytology, and urease test) were positive.
Fourteen patients were positive for H. pylori in group A, in comparison to seventeen
patients in group B. The sensitivities of the histopathological examination, brush
cytology, and urease test at 24 hours in group (A) were 58%, 79%, and 93%
respectively. Corresponding figures for the specificity were 100%, 91%, and 46%
respectively. While in group (B) the sensitivities were 82%, 82%, and 100% and the
specificities were 100%, 100%, and 88% respectively.
It is concluded that among the invasive methods, the association of the urease test
with brush cytology constituted the best choice for confirming the diagnosis of H.
pylori, due to the high sensitivity of the urease test and high specificity of brush
cytology.